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. 2014 May 7;9(5):e95993. doi: 10.1371/journal.pone.0095993

Figure 2. WP1066 inhibits proliferation and stimulates apoptosis of AGC cells.

Figure 2

Proliferation: A. AGS cells were treated with WP1066 at 5 µM for 18 hr, stained with trypan blue and viable cells counted. Data are expressed as the percentage of viable cells compared to vehicle alone. N = 3, mean±SE. B. CFSE tracking was used to measure changes in AGS cell proliferation after treatment with WP 1066. The raw fluorescence data collected is shown compared to DMSO controls for a representative experiment. Apoptosis: C. AGS cells treated for 24 hr with DMSO (method control), WP1066 (5 µM) or etoposide (200 µM; positive control) and adherent cells were stained with Annexin V then counted manually. Data are expressed as fold change compared to DMSO vehicle from a representative experiment. *p = 0.047.