Figure 5. WP1066 treatment of gp130^757FF mice inhibits the pro-inflammatory response in the antral mucosa.

gp130^757FF mice (8 weeks old) treated as for Fig. 3, had antral stomach tissue taken for histology and pro-inflammatory gene expression by Q-PCR after mRNA extraction. Treatment of gp130^757FF mice with WP1066 caused a significant reduction (p<0.05) in polymorphonuclear infiltrate (A) in the antral stomach, but lymphoplaysmocytic infiltrate was unchanged (B). Gene expression relative to the housekeeper GAPDH for IL-6 (C), IL-11 (D), IL-1 α (E), IL-1β (F) and COX-2 (G), was quantified by the ΔΔCT method. All n = 8; *p<0.05 compared to DMSO controls.