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. 2014 May 7;9(5):e96755. doi: 10.1371/journal.pone.0096755

Figure 4. L-carnosine reduces HIF-1α dependent gene transcription in HCT-116 cells.

Figure 4

(A) The table reports the primer sequences used in the Real Time PCR experiments; (B) aldolase A and PDK-1 mRNAS were measured by real time PCR in a total RNA preparation from HCT-116 cells 24 h after L-carnosine treatment. Bars indicate fold changes of mRNAS with respect to the amount of aldolase A and PDK1 mRNAs in untreated cells reported as value 1; (C), (D) and (E) western blotting analyses of total protein extracts from HCT-116 cells 24 h after L-carnosine treatment probed with aldolase A, Aldolase C, GLUT-1 and β-actin antibodies. Histograms report the densitometric values of aldolase A/β-actin ratio, aldolase C/β-actin ratio and GLUT-1/β-actin ratio. The densitometric values represent the mean ± SDs of three independent experiments. The differences were considered significant at **p<0.001, ***p<0.0001.