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. 2014 May 7;9(5):e96239. doi: 10.1371/journal.pone.0096239

Figure 3. Forced expression of anti-miR-363 and anti-miR-582-5p triggers apoptosis.

Figure 3

0822 and U373 GBM stem cell numbers for anti-miR-363 (grey squares), compared to scrambled anti-miRNA negative control (black diamonds) (A). 0822 and 0308 cell numbers for anti-miR-582-5p (grey squares), compared to scrambled anti-miRNA negative control (black diamonds) (C). Immortalized astrocyte and established GBM cell line A172 cell numbers following transfection of miR-582-5p (grey squares) compared to control miRNA (black diamonds), as well as for anti-miR-582-5p (grey squares) compared to anti-miRNA control (black diamonds) for immortalized astrocytes (C). Caspase 3/7 activity and/or Annexin V positivity (shown as % apoptosis) for anti-miR-363 in GBM stem cell lines 0308, 0822 and 1228, compared to scrambled anti-miRNA negative control (D). Caspase 3/7 activity for GBM stem cell lines for anti-miR-582-5p, compared to scrambled anti-miRNA negative control (E). p-values for Student’s t-test analyses are given as *, p<.05, **, p<.01, ***, p<.001. Experiments shown are representative of at least three separate experiments per cell type.