Table 1. PCR primers.

Gene	Forward	Reverse
ASCL2	CTCGACTTCTCCAGCTGGTTA	AGTGGAAGGTCTCTGCGGACA
BADa	TTATGCAAAACGAGGCTCGG	GGGTTAATCTCGGCTCGCAA
CAG-BADb	CCGACCGAAAGGAGCGCACGA	CTCATTTTATTAGGAAAGGACAG
HK (3):
Cyclophilin	GCATACAGGTCCTGGCATCT	TCTCCTGGGCTACAGAAGGA
GAPDH	CTCCCAACGTGTCTGTTGTG	TGAGCTTGACAAAGTGGTCG
HPRT	GCCGACCTGTTGGATTACAT	ACACTTCGAGGGGTCCTTTT

a These primers lie in the 3′ UTR of cattle BAD. As this region was not cloned into pCAG-BAD, they amplify only the endogenous BAD.

b Ectopic BAD expression as well as genotyping were performed with these primers (163 bp amplimer) which lie in the 3′ UTR of the pCAG vector.