Table 2. In vitro development to Day 7 of zona free nuclear transfer transgenic and singly cultured control IVP embryos.
Eggs | 2-Cell | Early dev (%)a | Pc | Late dev (%)b | Pc | |
pCag-BAD (line 1)d | 158 | 154 | 121 (79%) | 5.0E-13 | 48 (40%) | 0.25 |
pCag-BAD (line 2)d | 157 | 151 | 106 (70%) | 3.5E-08 | 45 (42%) | 0.45 |
IVPd | 210 | 184 | 73 (40%) | 36 (49%) |
a Number and percentage of cleaved embryos developing to at least morula stages;
b Number of Grade 1 or 2 blastocysts and as a percentage of those embryos having developed to at least morula stages;
c Significance of difference between tg lines and IVP embryos as determined by Fisher's Exact test.
d These embryos were grown concurrently.