Figure 4. (A) Mitotic localization of ATM, BRCA1, NuMA1, and TNKS1. HeLa cells were fixed and processed for immunofluorescence and confocal microscopy analysis. BRCA1, NuMA1, and TNKS1 were stained in red, ATM in green, and DNA in blue. Mitotic phases are indicated. (B) HeLa cells were fixed and processed for immunofluorescence. NuMA1 was stained in red, P-Ser1981-ATM in green, and DNA in blue. Scale bars, 10 μm.