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. 2014 Feb 11;13(7):1180–1186. doi: 10.4161/cc.28111

Figure 1.

Figure 1.

ZIP4 confers resistance to zinc deficiency-induced apoptosis in MIA PaCa-2 cells. (A) MIA PaCa-2 cells were treated with 2% FBS Chelex media for 48 h; washed twice in warm PBS, then prepared for 3′-biotinylation of fragmented DNA using the TUNEL assay procedure. Individual fields of cells were captured for both DNA 3′-end labeling (red) or total cellular DNA (blue). Representative images of 3 independent experiments were shown. Scale bar represents 500 µm. (B) Quantitative data of panel (A) **P < 0.0001. (C) MIA PaCa-2 cells were treated with 2% FBS Chelex media and non-Chelex media for 48 h; prepared for 3′-biotinylation of fragmented DNA using the TUNEL assay procedure; then fluorescence was acquired by BD flow cytometer. Representative histograms of 3 independent experiments were shown. Scale bar represents 500 µm. (D) Quantitative data of flow cytometry analysis. **P < 0.01.