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Silencing of ZIP4 induces cell cycle arrest in pancreatic cancer cells under zinc-deficiency condition. Cell cycle was analyzed by flow cytometry. Cells were starved for 48 h and then released by incubation with 2% RPMI media for 2 h. Then cells were stained with PI (BD Bioscience) before acquisition by a flow cytometer. The percentage of cells in G0/G1, S, and G2/M phases were measured and shown as mean ± SD of triplicate values. *P < 0.05.
