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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Arch Virol. 2013 Dec 1;159(5):1067–1077. doi: 10.1007/s00705-013-1932-z

Table 1. Genetic stability of the PB2-RSVF virus.

Percentage of plaques positive for RSV F protein*
Passage 1 Passage 2 Passage 3 Passage 4 Passage 5
Exp. 1 100% 100% 90% 100% 90%
Exp. 2 100% 100% 100% 90% 80%
Exp. 3 100% 90% 90% 100% 90%
*

The respective viral supernatants were subjected to standard virus plaque assays in confluent AX4/PB2 cells. Ten plaques were marked per well, which were then subjected to an immunodetection assay with an anti-RSV F protein antibody. The percentage of viral plaques that were positive for the RSV F protein among the 10 plaques is presented. The results of three independent experiments (Exp.) are shown.