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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Mol Cancer Ther. 2014 Feb 27;13(5):1246–1258. doi: 10.1158/1535-7163.MCT-13-0605

Figure 5. Pharmacological Jak1/2 inhibitor AZD1480 blocks IL-6-Jak-Stat3/ERK1/2-driven metastases formation in nude mice.

Figure 5

Genetic knockdown of Stat3 and/or ERK1/2, as well as pharmacological inhibition of Jak1/2 by AZD1480, suppresses PC metastases formation in nude mice. DU145 cells infected with lentivirus expressing IL-6 (MOI=5) with or without simultaneous infection of lentiviruses expressing shRNA-Stat3 (MOI=5) and/or shRNA-ERK1/2 (MOI=5) were injected in athymic nude mice through tail veins. AZD1480 was administered to indicated groups of mice daily by oral gavage (50 mg/kg). The lungs were stained with India Ink and scored for surface metastases. Representative photographs of India Ink-stained lungs (lower panel).