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Absorbance (A) and fluorescence (B) spectra of Au NP-Pc 227 conjugates in aqueous media, with overlapping spectra of both the NP (surface plasmon at 520 nm) and Pc 227 (690 nm absorbance) but with nearly complete quenching of fluorescence. NIR irradiation of Pc 227 in water (C & D) and chloroform (E & F) was determined through the new absorption band at ~671 nm and fluorescence at 680 nm.
