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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Mol Cancer Ther. 2014 Jan 16;13(5):1194–1205. doi: 10.1158/1535-7163.MCT-13-0341

Figure 2. Inhibition of Brd2 reduces STAT5 transcriptional function.

Figure 2

(A) ALL-SIL cells were transfected with a STAT5-luciferase construct (NCAM-luc) and treated with vehicle, JQ1 (1 μM), iBET (1 μM) or the enantiomer (−)-JQ1 (1 μM), after which STAT5-dependent gene expression was determined by dual luciferase assay. (B) DU528 cells were treated with vehicle, JQ1 (1 μM) or iBET (1 μM) for 6 hours, after which expression of the indicated STAT5 target genes was determined by quantitative RT-PCR. HEL cells (C, E) or ALL-SIL cells (D) were treated with the indicated shRNA constructs targeting BRD2, after which expression of the indicated genes was determined by quantitative RT-PCR.