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Dose-response effect of puromycin treatment on protein translation in vivo. Anti-HA (12CA5) and anti-puromycin (12D10) western blots of a galactose-induced 3xHA-Ade17p strain grown in 0mM-2mM puromycin for 15 minutes. No galactose induction was present for the sample marked –Gal, while the other samples were grown in 2% galacotse for 15 minutes prior to puromycin addition. Anti-actin is shown to demonstrate loading equivalency.
