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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Dev Biol. 2014 Mar 18;390(1):1–13. doi: 10.1016/j.ydbio.2014.03.004

Figure 2. Generation of Foxi3 mutant mice.

Figure 2

A: Diagram of the mouse Foxi3 locus, composed of two coding exons. A targeting vector was generated containing a loxP site upstream of exon 2 and an Frt-PGKNeo-Frt-LoxP sequence downstream of exon. B: The successfully targeted allele introduced an extra NheI and EcoRV site into the locus that could be detected by Southern blotting with 5’ and 3’ probes. Crossing successfully targeted offspring with CMV-Cre mice recombined the loxP sites to generate the Foxi3-del allele,