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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Dev Biol. 2014 Mar 18;390(1):1–13. doi: 10.1016/j.ydbio.2014.03.004

Figure 4. Foxi3 mutant mice do not form pharyngeal pouches.

Figure 4

(A) Coronal sections through 12ss embryos stained with DAPI reveal absence of a distinct pharyngeal pouch 1 (p1) between the first two pharyngeal arches (a1, a2) in the Foxi3 mutant (asterisk). At 25 somites, confocal imaging of DAPI-stained embryos shows that Foxi3 mutant embryos have clearly failed to form distinct arches (asterisk). (B) Whole mount embryos at 15 somites and 25 somites show less distinct patterns of Pax9 expression around the presumptive pharyngeal pouches. Coronal sections through these embryos reveal continuous expression of Pax9 along the entire extent of pharyngeal endoderm at both ages in Foxi3 mutants, whereas Pax9 is restricted to pouches in wild type embryos.