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. Author manuscript; available in PMC: 2014 May 7.
Published in final edited form as: Cell Rep. 2013 Aug 29;4(5):879–889. doi: 10.1016/j.celrep.2013.07.042

Figure 1. A targeted RNAi screen of XLID genes in ciliary morphogenesis in neurons.

Figure 1

Hippocampal neurons were transfected with an RNAi plasmid encoding shRNAs targeting the indicated XLID gene or the control U6 plasmid together with the GFP expression plasmid and subjected to immunocytochemistry at DIV5 using the AC3 and GFP antibodies. Knockdown of each protein by cognate shRNAs was validated (Figures S1 and 2A). Knockdown of PQBP1 consistently reduced the percentage of neurons harboring a primary cilium (p < 0.05; ANOVA).

Total of 2646 neurons were quantified.

See also Figure S1