Table 1. Primers for CTGF, RhoG, ITGB1, and ROCK1 luciferase reporter constructures.
Gene | Forward primer sequence | Reverse primer sequence |
CTGF | 5′ -CGACTAGTGCCAGAGAGTGAGAGACATTAAC-3′ | 5′-TGAACGATCAGACAAGCTTTAC-3′ |
RhoG | 5′-TTACTAGTCCCTGGCACTTGGCTTGGA-3′ | 5′-TAGAAGCTTGAGTCAGTCAGCAAATGCGT-3′ |
ITGB1 | 5′ -TAGAGCTCCCGTGCAAATCCCACAACA-3′ | 5′-ATACGCGTTACATCAGAGTCAAGACATCCG-3′ |
ROCK1 | 5′-TTGAGCTCGTGCCCTGTGGAATCGTG-3′ | 5′-TCACGCGTTTATGTTGGTGCAACCTTCTA-3′ |
CTGF mutant | 5′-TTAAAGTTGTTTCTCCGTCTTTATTnTG-3′ | 5′-CAAAAATAAAGACGGAGAAACAACTTTAA-3′ |
RhoG mutant | ||
Site-1 | 5′-CCCACCAGTTATACATAGGTGCCTTGTCC-3′ | 5′-GGACAAGGCACCTATGTATAACTGGTGGG-3′ |
Site-2 | 5′-TCCGCCTCAGCTATACATAAAGGACTAATTC-3′ | 5′-GAATTAGTCCTTTATGTATAGCTGAGGCGGA-3′ |
Site-3 | 5′-CTTTTTCTCTGAATACATATTTCTCCTTAAG-3′ | 5′-CTTAAGGAGAAATATGTATTCAGAGAAAAAG-3′ |
ITGB1 mutant | ||
Site-1 | 5′-TAAGGTCACATTCTCCGTCTTTGACCTTTTC-3′ | 5′-GAAAAGGTCAAAGACGGAGAATGTGACCTTA-3′ |
Site-2 | 5′-ACATTCTTGTTTTAACTCCGTCTAGTTTTAACAG-3′ | 5′-CTGTTAAAACTAGACGGAGTTAAAACAAGAATGT-3′ |
ROCK1 mutant | 5′-ATTGTCCTTTTACTCCGTCAATTTGAGAT-3′ | 5′-ATCTCAAATTGACGGAGTAAAAGGACAAT-3′ |
CTGF, connective tissue growth factor; RhoG, ras homolog gene family member G; ITGB1, integrin-β1; ROCK1, Rho-associated coiled-coil containing protein kinase 1.