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. 2011 Dec;30(12):821–830. doi: 10.5732/cjc.011.10289

Table 1. Primers for CTGF, RhoG, ITGB1, and ROCK1 luciferase reporter constructures.

Gene Forward primer sequence Reverse primer sequence
CTGF 5′ -CGACTAGTGCCAGAGAGTGAGAGACATTAAC-3′ 5′-TGAACGATCAGACAAGCTTTAC-3′
RhoG 5′-TTACTAGTCCCTGGCACTTGGCTTGGA-3′ 5′-TAGAAGCTTGAGTCAGTCAGCAAATGCGT-3′
ITGB1 5′ -TAGAGCTCCCGTGCAAATCCCACAACA-3′ 5′-ATACGCGTTACATCAGAGTCAAGACATCCG-3′
ROCK1 5′-TTGAGCTCGTGCCCTGTGGAATCGTG-3′ 5′-TCACGCGTTTATGTTGGTGCAACCTTCTA-3′
CTGF mutant 5′-TTAAAGTTGTTTCTCCGTCTTTATTnTG-3′ 5′-CAAAAATAAAGACGGAGAAACAACTTTAA-3′
RhoG mutant
 Site-1 5′-CCCACCAGTTATACATAGGTGCCTTGTCC-3′ 5′-GGACAAGGCACCTATGTATAACTGGTGGG-3′
 Site-2 5′-TCCGCCTCAGCTATACATAAAGGACTAATTC-3′ 5′-GAATTAGTCCTTTATGTATAGCTGAGGCGGA-3′
 Site-3 5′-CTTTTTCTCTGAATACATATTTCTCCTTAAG-3′ 5′-CTTAAGGAGAAATATGTATTCAGAGAAAAAG-3′
ITGB1 mutant
 Site-1 5′-TAAGGTCACATTCTCCGTCTTTGACCTTTTC-3′ 5′-GAAAAGGTCAAAGACGGAGAATGTGACCTTA-3′
 Site-2 5′-ACATTCTTGTTTTAACTCCGTCTAGTTTTAACAG-3′ 5′-CTGTTAAAACTAGACGGAGTTAAAACAAGAATGT-3′
ROCK1 mutant 5′-ATTGTCCTTTTACTCCGTCAATTTGAGAT-3′ 5′-ATCTCAAATTGACGGAGTAAAAGGACAAT-3′

CTGF, connective tissue growth factor; RhoG, ras homolog gene family member G; ITGB1, integrin-β1; ROCK1, Rho-associated coiled-coil containing protein kinase 1.