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. 2013 Nov 1;76(3):323–330. doi: 10.1292/jvms.13-0419

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©2014 The Japanese Society of Veterinary Science

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Fig. 3. — Characterization of native BboDHODH. (A) SDS-PAGE showing purified BboDHODH-His stained with amide black. M: Low molecular marker; lane 1: purified rBboDHODH-His. (B) Western blot analysis showing reaction of anti rBboDHODH mice sera with parasite lysates. Lane 1: non infected bovine RBC lysates; lane 2: B. bovis lysates; lane a: anti-rBboDHODH mice sera; lane b: non-immunize mice sera. The arrowhead shows BboDHODH protein. (C) Localization of BboDHODH by IFAT. (1) Phase-contrast; (2) MitoTracker (red); (3) Anti-rBboDHODH (green), (4) The overlaid of fluorescence reactivity.