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In vitro inhibition assay. (A-1) Growth of B. bovis parasites was inhibited by treatment by atovaquone (ATV) and (A-2) leflunomide (LFN) from 48 hr post treatment. (B) Morphological change of parasites after treatment with ATV and LFN, respectively. (C) Viability of parasite after treatment by ATV, LFN and Di, respectively, at the significant inhibitory concentration (observed at day 10 after drug withdrawing).
