Table 2. Relative activity of rBboDHODH protein in presence of different DHODH Inhibitors.

Inhibitor	Relative activity (%)
ATV (1 µM)	19.10
LFN (1 mM)	51.58
Breq (0.1 mM)	106.50
TAZ (1 mM)	101.00
Without inhibitor	100.00

The reaction was measured using DCIP reduction assay, 200 µl-reactions contained 0.1 mM DCIP, 1 mM L-DHO, 0.1 mM Q_D and 0.205 µg of the recombinant protein. Relative activity of rBboDHODH in presence of inhibitor was compared to reaction of the enzyme without inhibitor which is taken as 100%.