Table 1. Binding specifities of lectins used in this study.
Lectins | Abbreviation | Concentration (mg/ml) | Binding specificity |
---|---|---|---|
Wheat germ agglutinin | WGA | 1.0 × 10ˉ2 | GlcNAc, NeuAc |
Succinylated-wheat germ agglutinin | s-WGA | 1.0 × 10ˉ2 | (GlcNAc) n |
Lycopersicon esculentum lectin | LEL | 2.0 × 10ˉ2 | (GlcNAc) 2–4 |
Solanum tuberosum lectin | STL | 1.0 × 10ˉ2 | (GlcNAc) 2–4 |
Datura stramonium lectin | DSL | 4.0 × 10ˉ3 | (GlcNAc) 2–4 |
Bandeiraea simplicifolia lectin-II | BSL-II | 4.0 × 10ˉ3 | GlcNAc |
Dolichos biflorus agglutinin | DBA | 1.0 × 10ˉ2 | Gal, GalNAc |
Soybean agglutinin | SBA | 1.0 × 10ˉ2 | Gal, GalNAc |
Bandeiraea simplicifolia lectin-I | BSL-I | 4.0 × 10ˉ3 | Gal, GalNAc |
Vicia villosa agglutinin | VVA | 4.0 × 10ˉ3 | Gal, GalNAc |
Sophora japonica agglutinin | SJA | 2.0 × 10ˉ2 | Gal, GalNAc |
Ricinus communis agglutinin-I | RCA-120 | 2.0 × 10ˉ3 | Gal, GalNAc |
Jacalin | 5.0 × 10ˉ4 | Gal, GalNAc | |
Peanut agglutinin | PNA | 4.0 × 10ˉ3 | Gal |
Erythrina cristagalli lectin | ECL | 2.0 × 10ˉ2 | Gal, GalNAc |
Ulex europaeus agglutinin-I | UEA-I | 2.0 × 10ˉ2 | Fuc |
Concanavalin A | ConA | 3.3 × 10ˉ3 | Man, Glc |
Pisum sativum agglutinin | PSA | 4.0 × 10ˉ3 | Man, Glc |
Lens culinaris agglutinin | LCA | 4.0 × 10ˉ3 | Man, Glc |
Phaseolus vulgaris agglutinin-E | PHA-E | 5.0 × 10ˉ3 | Oligosaccharide |
Phaseolus vulgaris agglutinin-L | PHA-L | 2.5 × 10ˉ3 | Oligosaccharide |
Fuc, fucose; Gal, galactose; GalNAc, N-acetylgalactosamine; Glc, glucose; GlcNAc, N-acetylglucosamine; Man, mannose; NeuAc, N-acetylneuraminic acid.