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. 2011 Jul;30(7):472–481. doi: 10.5732/cjc.011.10157

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Chinese Journal of Cancer

This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License, which allows readers to alter, transform, or build upon the article and then distribute the resulting work under the same or similar license to this one. The work must be attributed back to the original author and commercial use is not permitted without specific permission.

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Figure 3. — A, the process of motor neuron differentiation. Embryonic stem cells in embryonic bodies can be induced by retinoic acid (RA) and SMO agonist purmorphamine (AG) to express Hh target genes GM and FoxA2 as well as expression of motor neuron differentiation markers oligo2 and Hb9. The whole process takes about 5 days. B, immunofluorescent staining with Hb9 antibody in embyonic bodies of the ES14Tg2a mouse embryonic stem cell line. Hb9 expression was induced by incubation with RA and AG and inhibited by CycT. C, real-time PCR data on motor neuron differentiation. Expression of Hh target genes Hip, GM, and FoxA2, as well as neuron markers 0lig2 and Hb9 were examined. In the presence of CycT, no induction of Hh target genes or motor neuron markers was observed.