Figure 4. Effect of CycT on skin lesions from Krt6a-cre: Ptch1^neo/neo mice.

Following drug treatment, mouse skin biopsies from the areas with drug administration were collected for HE staining. The effectiveness of CycT was examined by comparing the tumor areas between the two groups (A) and ImageJ analyses of 8 independent tissue areas per mouse (B, the average data from 6 mice in each group). While the skin in the control group contained ∼30% of tumor area in the skin section, CycT-treated skin had only 10% to 15% of tumor area in the tissue. With 6 mice for each group, the statistical power for this study was 90.6. The level of Hh target gene expression was examined by real-time PCR (C), with both GM and Hip being down-regulated by CycT (P < 0.05 from unpaired Student's t-test for two samples).