Table 1.
Main cohort of 16 microdissected MPCs subjected to microarray-based comparative genomic hybridization and Sequenom MassARRAY OncoCarta analysis. Five of the cases were subjected to RNA massively parallel sequencing due to the availability of a frozen specimen
| Sample | Pure/mixed | Specimen type | Grade | ER | PR | HER2 | RNA-seq | Mutation* |
|---|---|---|---|---|---|---|---|---|
| MPC10 | Pure | Frozen | 3 | Positive | Positive | Negative | Y | None |
| MPC08 | Pure | Frozen | 2 | Positive | Positive | Negative | Y | None |
| MPC70 | Pure | Frozen | 2 | Positive | Positive | Negative | Y | None |
| MPC72 | Pure | Frozen | 3 | Positive | Negative | Negative | Y | None |
| MPC71 | Pure | Frozen | 3 | Positive | Positive | Positive | Y | None |
| MPC37 | Mixed | Frozen | 3 | Positive | Positive | Negative | NP | None |
| MPC06 | Pure | FFPE | 2 | Positive | Positive | Negative | NP | None |
| MPC11 | Pure | FFPE | 2 | Positive | Positive | Negative | NP | None |
| MPC02 | Pure | FFPE | 2 | Positive | Positive | Negative | NP | None |
| MPC04 | Pure | FFPE | 3 | Positive | Negative | Negative | NP | None |
| MPC01 | Pure | FFPE | 2 | Positive | Positive | Negative | NP | None |
| MPC23 | Pure | FFPE | 3 | Positive | Negative | Negative | NP | None |
| MPC25 | Mixed | FFPE | 2 | Positive | Positive | Negative | NP | PIK3CA H1047R |
| MPC45 | Mixed | FFPE | 3 | Positive | Positive | Positive | NP | None |
| MPC38 | Mixed | FFPE | 3 | Positive | Positive | Negative | NP | None |
| MPC53 | Mixed | FFPE | 3 | Negative | Negative | Positive | NP | None |
ER, oestrogen receptor; FFPE, formalin-fixed, paraffin-embedded; NP, not performed; PR, progesterone receptor; RNA-seq, paired-end massively parallel mRNA sequencing; Y, yes.
*
Mutations in the hotspot regions of 19 genes assessed by the Sequenom OncoCarta v1.0.