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Series of parasagittal sections through the hypothalamus of an E18.5 embryo at lateral (a–l) and intermediate (m–r) levels, correlating the expression pattern of the indicated reference markers (Otp, Dlx5) with the presence of Trh+ cells. Corresponding digital overlaps with pseudocolored reference markers and Trh signals are marked with a plus symbol. Scale bars 300 μm
