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. 2014 May 1;5:170. doi: 10.3389/fphys.2014.00170

Figure 2.

Figure 2

Characterization of G-CSF and G-CSFR during C2C12 myogenesis. (A) Representative images of C2C12 during differentiation. (B) Expression of myosin heavy chain genes (Myh7, 2, 4, 1) during following differentiation of C2C12 cultures. (C) G-CSF (Csf3) and G-CSFR (Csf3r) mRNA expression and (D) G-CSFR protein expression during differentiation of C2C12 cultures. Day 0 represents near confluent myoblasts (>90% confluent). Day 1 represents 24 h post serum withdrawal. mRNA is expressed as a fold change after Day 0 (n = 3). *p < 0.05, **p < 0.01 and ***p < 0.001.