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. 2014 May 1;5:189. doi: 10.3389/fimmu.2014.00189

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Copyright © 2014 Nico, Gomes, Alves-Silva, Freitas, Morrot, Bahia, Palatnik, Rodrigues and Palatnik-de-Sousa.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Long-term cross-protection generated by the F3sap and F1sap vaccines. Balb/c mice were vaccinated with three doses of F1sap or F3sap with a weekly interval and challenged with L. amazonensis infective promastigotes, 30 days after the last immunization. The evolution of the sizes of footpad lesions (A) and the parasite load in lesions (limiting dilution assay) (B) were determined. Bars represent the mean ± SD of one experiment with 10 mice for each treatment. *p < 0.05 significant differences from the saline controls and ∘ from the F1sap vaccine.