FIG 6.

A, Dust mite antigens can bind to inflammatory cells in polyp tissues through IgE. Representative immunostaining of consecutive sections of polyp tissues with biotinylated Dermatophagoides farinae antigens with or without anti-IgE pre-treatment, respectively. The staining is almost completely abolished by pretreatment with anti-IgE antibody. Sections were from an eosinophilic chronic rhinosinusitis with nasal polyps (CRSwNP) subject with local production of specific IgE to duct mites. B, Many cells bound with dust mite antigens are tryptase positive mast cells. Representative immunostaining of consecutive sections of polyp tissues with biotinylated dust mite allergens and anti-tryptase antibody, respectively. Sections were from the same eosinophilic CRSwNP subject. C, A representative photomicrograph of immunohistochemical staining showing negative control for mite antigen binding. Arrows with the same direction indicate same cells in consecutive serial sections, original magnification × 400.