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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Circ Cardiovasc Genet. 2014 Jan 6;7(1):23–32. doi: 10.1161/CIRCGENETICS.113.000259

Figure 4.

Figure 4

Validation of potential Pitx2 targets from ChIP-Seq and microarray assays. (A) Genome browser tracks for potential targets of Pitx2 in adult heart identified by ChIP-Seq. Peaks from ChIP-Seq, conservation with human genome and the chromosomal regions used in reporter assay are shown. Normalized ChIP-Seq tag numbers are shown on the Y-axis. (B) Alteration in expression levels for the potential Pitx2 targets were detected by realtime RT-PCR in the left atrium of 3 to 4-month-old control and Pitx2 conditional knock out mice. (n=6) (C) Reporter assay for ChIP-Seq identified potential targets of Pitx2. Chromosomal regions were named after the genes in closest proximity. Values and error bars represent mean and standard deviation (n=6).