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IL-2-dependent activation-induced cell death is mediated by caspase-3. Day 5 T-cell blasts from wild-type or caspase-3-deficient mice were cultured with or without anti-CD3 for 4 h and assessed for (a) caspase activity or (b and c) proportion of dead cells by TUNEL staining. Values are mean±S.E.M. The significance of differences observed in caspase activity was analyzed by two-way ANOVA followed by Bonferroni multiple comparisons test (*P<0.0001)
