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. 2014 Feb 7;21(6):904–914. doi: 10.1038/cdd.2014.10

Figure 3.

Figure 3

IL-15 regulates caspase-3 activity independently of caspase-8 and caspase-9. (a) Day 5 IL-2- and IL-15-cultured T cells were lysed in buffer containing biotin-VAD (bVAD) to selectively label active caspases. Then, 450 μg protein lysate was selectively precipitated with streptavidin-sepharose beads. Precipitates were resolved by SDS-PAGE and immunoblotted for caspase-8, caspase-9, and caspase-3, and compared with whole-cell lysates (WCLs; 30 μg) from the same cells. (b–d) Densitometry of bVAD precipitates immunoblotted for (b) caspase-8, (c) caspase-9, and (d) caspase-3. Values are the combined readings of three independent experiments. Values are mean±S.E.M. Data were analyzed by Student's t-test for IL-2- versus IL-15-cultured T cells (*P<0.05)