Figure 7.

Increased reactive species in IL-15-cultured T cells. Day 5 wild-type T cells cultured in the presence of either IL-2 (50 U/ml) or IL-15 (20 ng/ml) were analyzed for (a) ROS measured by DCFDA and flow cytometry. Data are representative of three independent experiments. (b) Nitric oxide as a measurement of total nitrate+nitrite by DAN probe. (c) Reduced glutathione (GSH), (d) oxidized glutathione (GSSG), or (e) GSH+GSSG were independently assayed. Data are based on three independent experiments combined. Values are mean±S.E.M. The significance of differences observed was analyzed by Student's t-test (*P<0.05; **P<0.001)