Figure 6.

TAZ overexpression induces osteogenic genes transcription, which was decreased by TAZ depletion. (a) Re-introduction of TAZ in C3H10T1/2 cells was assessed by a retrovirus producing Flag-TAZ. Bp and Ti indicate vector control cells and TAZ knockdown cells, respectively. Ti-TAZ indicates Flag-TAZ introduced TAZ knockdown cells. TAZ expression was analysed by western blot. (b) Bp, Ti and Ti-TAZ cells were differentiated in osteogenic differentiation medium. At 2 days after differentiation, alkaline phosphatase activity was analysed to determine the osteogenic potential of each cells. A blue colour indicates increased alkaline phosphatase activity. (c) The cells in (a) were induced to osteoblastic differentiation with or without Wnt3a-conditioned media. At 2 days after differentiation, total RNAs were collected from the cells, and OC, OSX, MSX2, TAZ and Runx2 expression levels were analysed by qRT-PCR. Their relative expression was determined after normalization to the GAPDH level