Figure 5.

IRF4 protects neurons from OGD-induced cell death. (a–d) Quantification of neuronal death following OGD at the indicated time points by cell viability (a, c) and LDH release (b, d). The primary neurons were infected with AdshIRF4 (a, b), AdIRF4 (c, d) or control adenoviruses. For each time point, three independent experiments were performed in triplicate. *P<0.0001 (a, b) compared with AdshRNA, unpaired Student's t-test; *P=0.0004 (c, 6 h), <0.0001 (c, 12 and 24 h) and <0.0001 (d) compared with AdGFP, unpaired Student's t-test. (e and f) Immunoblotting of Bcl2 and cleaved caspase-3 in primary neurons infected with AdshIRF4 (e) or AdIRF4 (f) before OGD for the indicated times. GAPDH served as a loading control in each lane. The right panels show the quantification of the indicated protein levels normalised to GAPDH. For each time point, three independent experiments were performed in triplicate. *P<0.0001 versus AdshRNA in e, unpaired Student's t-test; *P<0.0001 versus AdGFP in f, unpaired Student's t-test. The values are the means±S.E