. Author manuscript; available in PMC: 2014 May 8.

Published in final edited form as: Anal Chem. 2013 Jun 6;85(12):6011–6017. doi: 10.1021/ac400831z

Table 1.

Quantification of UCH-L1 in the High-Speed Supernatant and Pellet

donor ID no.	UCH-L1 protein (pmol/mg tissue protein)^a
	control								AD
	1	5	9	12	13	14	15	18	2	3	6	7	8	10	11	16	17	19
Supernatant
Q1 consensus	245 ± 10	185 ± 12	235 ± 3	253 ± 16	198 ± 8 224 ± 31	192 ± 5	214 ± 16	269 ± 18	181 ± 16	67 ± 13	211 ± 3	168 ± 2	83 ± 1	130 ± 7 131 ± 50	121 ± 19	121 ± 10	65 ± 6	165 ± 13
Q3 consensus	342 ± 23	277 ± 18	387 ± 16	335 ± 13	280 ± 16 311 ± 63	183 ± 7	328 ± 3	354 ± 20	138 ± 20	76 ± 15	199 ± 21	233 ± 18	178 ± 6	164 ± 8 167 ± 56	162 ± 6	213 ± 15	77 ± 4	225 ± 11
Pellet
Q1 consensus	87 ± 2	83 ± 18	125 ± 4	81 ± 19	72 ± 6 90 ± 22	103 ± 9	57 ± 6	112 ± 17	62 ± 11	85 ± 12	113 ± 3	108 ± 10	110 ± 1	81 ± 4 102 ± 26	109 ± 11	100 ± 10	160 ± 4	94 ± 5
Q3 consensus	117 ± 7	111 ± 12	105 ± 14	115 ± 12	140 ± 16 119 ± 28	175 ± 8	78 ± 8	113 ± 4	75 ± 7	118 ± 10	138 ± 18	141 ± 20	117 ± 5	154 ± 9 136 ± 36	183 ± 17	150 ± 14	191 ± 6	96 ± 10

Measurements were performed on frontal cortex high-speed supernatant and pellet from control and severe AD human donors (Supporting Information, Table S1). The concentration was calculated for three experimental replicates by monitoring three transitions per individual Q1 and Q3 peptides. For the consensus, the data based on Q1 or Q3 peptides were combined for each control and AD groups. All data are presented as mean ± SD. The monitored transitions are summarized in the Supporting Information, Table S2.