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. 2014 Apr 4;15(4):5838–5851. doi: 10.3390/ijms15045838

Figure 3.

Figure 3.

Knockdown of MET by siRNA in Caco-2 cells restores sensitivity to cetuximab. (A) Caco-2 cells were transiently transfected with two pairs of MET siRNA separately and Scramble Control siRNA for 48 h. Knockdown of MET and successive inhibition of downstream targets were examined by Western blotting; (B,C) Caco-2 cells were transfected with Scramble Control siRNA or MET siRNA followed by treatment with 10 μg/mL cetuximab for 48 h in 2% FBS medium. Cell lysates were subjected to Western blotting, and cell viability was assessed by MTT assay. The results from three independent experiments are shown. Student’s t-test, * p < 0.01, ** p < 0.001 for comparisons between two arms.