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. 2014 Apr 15;15(4):6343–6355. doi: 10.3390/ijms15046343

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© 2014 by the authors; licensee MDPI, Basel, Switzerland

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Figure 1. — 2D reference images of four Fe/Cd combined treatments: (A) Sufficient in iron and no cadmium (+Fe/−Cd, control); (B) Deprived iron and cadmium toxicity (−Fe/+Cd); (C) Deprived iron with no cadmium (+Fe/−Cd); and (D) Sufficient iron but cadmium toxicity (+Fe/+Cd) in the roots of Vigna radiata L. Proteins were separated in the first dimension on a nonlinear IPG strip, pH 4–7 and in 2nd dimension on 12% polyacrylamide SDS-gel. Quantitative image represents differentially expressed proteins. Red encircles indicate absence of protein spots with respect to that of control. Black arrows indicate comparison of protein spots to each other identified by MALDI-TOF.