Figure 2.

The temperature dependence of trastuzumab stability upon storage for 7, 14, 31 and 45 days. Method: 2-DE (3–11 NL) with silver staining. Trastuzumab, 10 μg. Storage conditions: gels on the left, ambient temperature; gels on the right, 37 °C: Control, 4–8 and 50 °C (in the center of the figure). Compared to the control gel, the change in the spot pattern can be noted only after longer storage times (i.e., 31 and 45 days), particularly in samples subjected to elevated temperatures. Numerous higher molecular spots have been detected in samples stored at 50 °C.