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. 2014 Apr 17;15(4):6544–6555. doi: 10.3390/ijms15046544

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© 2014 by the authors; licensee MDPI, Basel, Switzerland

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Figure 1. — Expression levels of miR-196a and miR-196b in human osteosarcoma tissues and patients’ sera detected by qRT-PCR (Quantitative real-time reverse transcriptase-polymerase chain reaction) assay. The results showed that the expression levels of miR-196a (A) and miR-196b (B) in osteosarcoma tissues were both significantly higher than those in noncancerous bone tissues (both p < 0.001); Similarly, the serum levels of miR-196a (C) and miR-196b (D) were also markedly upregulated in patients with osteosarcomas compared with healthy controls (both p < 0.001); More interestingly, the expression levels of miR-196a and miR-196b in osteosarcoma tissues were both significantly correlated with those in patients’ sera (for miR-196a: Spearman’s correlation: r = 0.62, p = 0.01, (E); for miR-196b: Spearman’s correlation: r = 0.68, p = 0.001, (F)).