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. 2014 Apr 17;15(4):6592–6608. doi: 10.3390/ijms15046592

Figure 1.

Figure 1.

Figure 1.

Changes in the protein expression of A20 in RPMCs after exposure to LPS. (A) RPMCs were treated with 1, 5, 10, and 20 μg/mL LPS for 4 h, respectively. Untreated cells were used as the control. The relative expression of A20 protein was determined by normalization to β-actin. Western blots are representative of three separate experiments. Bar graph shows the relative expression of A20. Data are presented as the mean ± SD (n = 3). * p <0.01 and # p < 0.005 vs. control; (B) Changes in the protein expression of A20 in RPMCs after exposure to LPS. RPMCs were treated with 1 μg/mL LPS for various times (2, 4, 8, 12, and 24 h). Untreated cells were used as the control. The relative expression of A20 protein was determined by normalization to β-actin. Western blots are representative of three independent experiments. Bar graph shows the relative protein expression of A20. Data are presented as the mean ± SD (n = 3). * p < 0.05 and # p < 0.01 vs. control; (C) The protein expression of A20 in RPMCs in the absence (A20−) or presence (A20+) of pGEM-T easy-A20 (1 μg/well). RPMCs were transfected with pGEM-T easy or pGEM-T easy-A20 for 24, 48, and 96 h, respectively. Cells incubated with serum-free DMEM/F12 alone were used as the control. The relative expression of A20 protein was determined by normalization to β-actin. Western blots are representative of three separate experiments. Bar graph shows the relative protein expression of A20. Data are presented as the mean ± SD (n = 3). * p < 0.001 vs. control.