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. 2014 Apr 17;15(4):6641–6656. doi: 10.3390/ijms15046641

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© 2014 by the authors; licensee MDPI, Basel, Switzerland

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Figure 5. — Effect of farrerol on 60 mM KCl-induced elevation of [Ca²⁺]_in in rat aortic VSMCs. [Ca²⁺]_in profiles of VSMCs following addition of farrerol assayed by Fluo 4-AM using a laser scanning confocal microscope at room temperature. The fluorescence intensities change was plotted to represent the change in [Ca²⁺]_in levels. (A–E) Original fluorescence images (×400). (A) before KCl was added (control); (B) 60 mM KCl was added; (C) wash out KCl with physiological salt solution; (D) 60 mM KCl was added; (E) 14.02 μM farrerol was added; and (F,G) representative original tracing of relative fluorescence intensity of [Ca²⁺]_in in each treatment (arrow indicates the time point when 60 mM KCl or 14.02 μM farrerol was added).