Table 4.
Sequences of primers used for qRT-PCR.
| Target gene | Primers | Sequence (5′ to 3′) |
|---|---|---|
| SETD2 | SETD2qPCRF1 | CCTACAGGACATCTGGAGTTAC |
| SETD2qPCRR1 | GAATCAGTACCAGCATTTAGATG | |
| DOT1L | dot1qPCRF1 | AGAATCCGAACGACTCGACAG |
| dot1qPCRR1 | CTGTTCTTGGTCTTCGTTCAAC | |
| EZH2 | EZH2F1 | GGTGTAGTGACAACCCAACAG |
| EZH2R1 | TCTTAACTCCTGAGCTGTTCC | |
| ASH2 | ASHF2 | GGGGACCAGGTTCCACGAGTC |
| ASHR2 | TACAGGTGGCTCTTGAATTTG | |
| Bmdsx promoter | BmdsxproF1 | TGCATGTTTCTTATTAATCAGCTAG |
| BmdsxproR1 | GTAAATTTCGTAAAAGCTGACCAG | |
| Bmdsx E1 | ChIPEx1F | CCTGTACCACCAGTGGTGAAG |
| ChIPEx1R | CTGACGGCGGTGGAGCGTATG | |
| Bmdsx I2 | ChIPI2F | CTACATGAACAGTACCAGTCAG |
| ChIPI2R | GTAAGTACAAACTAAATAGCGTTC | |
| Bmdsx E3 | ChIPEx3F | GTCGACGAGTACGCGAGGAAG |
| ChIPEx3R | TGTGATGCATGTATCTGTCGC | |
| Bmdsx I3 | ChIPI3F | GTAACTGACCTTCTTGCTAATC |
| ChIPI3R | CTGTGCCATTTTATTAATATCGTC | |
| Bmdsx E4 | ChIPEx4F | ATATAAGTGGTGTACTGTCTTC |
| ChIPEx4R | CCATAGATCCAATGTTACGAC | |
| Bmdsx I4 | ChIPI4F | GTTCAAACACATCGAAGCTAC |
| ChIPI4R | GTCCGAGATAGACTGGCCTTG | |
| Bmdsx E6 | ChIPEx6F | GGCACAGCGCCGACAAGTAAG |
| ChIPEx6R | ATTGTCTGTAGATATTCGTGATC | |
| BmIMP promoter1S | IMPproF2 | TTAAGCATTTAATTATAAGAAGATC |
| IMPproR2 | CTAGAATCTGCGATTACATAC | |
| BmIMP E1S | IMPE1F1 | TCCGTTCAGTACTCGCTATAC |
| IMPE1R1 | TCTTACCTATCGTCATAGATTC | |
| BmIMP I1S | IMPI1F2 | ATTTGGTAAAATAGTCTCGTATC |
| IMPI1R2 | ACCTTGTGATACGGGGTTAAC | |
| BmIMP pro1L | IMPE1LproF1 | GCTGCCCCACCCTTTAAACCG |
| IMPE1LproR1 | CTCGATCGTGCTGACTCTAGC | |
| BmIMP E1L | IMPE1F1 | TTTCAAGTATACTCCTTCTATAG |
| IMPE1R1 | TTCGCCATTTTGAGCAGATTG | |
| BmIMP I1L | IMPI1F3 | CAAATGGGCACATATTGTTGG |
| IMPI1R3 | GTTTAAGCGCTTTCGTGATGG | |
| BmIMP E7 | IMPE7F1 | ATGCGGGAAGAAGGTTTTATG |
| IMPE7R1 | AATGTGAACGGTGGTCTCGTG | |
| BmIMP I7 | IMPI7F1 | GTGCATAAATCCACAGAACAG |
| IMPI7R1 | TTACTCAGAAACTCAGAAGTAC | |
| BmIMP E8 | IMPE8F1 | CGTCTGAGGCGGGATGAGAAC |
| IMPE8R1 | TAAATTCGCCGCAATCAGCAG | |
| EF1 promoter | EF-1proF1 | TATATCAATTTTGGTGCAAGAATGG |
| EF-1proR1 | GTAATAATATTCTATTCTATCCACCG | |
| EF-1 E2 | EF-1E2F1 | TGGCGATGGAGGCGGAGAAG |
| EF-1E2R1 | CTCAACTTCCCAGCTGTCTGC | |
| EF-1 I3 | EF-1I3F1 | ACTTACTTATTTATGATCATGCGTC |
| EF-1I3R1 | GCTAACCACAATTATATTTGTGGAG | |
| EF-1 E6 | EF-1E6F1 | TACAGGTCATTTCTGCACGTAAG |
| EF-1E6R1 | TCATCCCAGTTAACTGTTGGATC | |
| EF-1 I11 | EF-1I11F1 | TTCATGGACTACATTTTACCTTGG |
| EF-1I11R1 | CTAAGCTCTTCTAAAAGAGATGAGC | |
| EF-1 I12 | EF-1I12F1 | GCATTAATATTAATTCCACCACAAG |
| EF-1I12R1 | CACACCTCACTGCTCTTCCGC | |
| Bm-Sxl promoter | Sxli1F1 | GGCTAAACTATCTTCAACAAG |
| Sxli1R1 | CGGTCACCGTTCTCGTGAAAG | |
| Bm-Sxl E1 | Sxle1F1 | GCCAGTCCAAATGGACGAATC |
| Sxle1R1 | GTTCACTGACTTTCGAGTGAG | |
| Bm-Sxl E2 | Sxle2F1 | GCCTACTCGAACAATAAAAAAG |
| Sxle2R1 | TTCCAAAGAATTGAAACTCCTG | |
| Bm-Sxl I2 | Sxli2F1 | TGAATCAGAACATCTCATTTGG |
| Sxli2R1 | CCAAGCCGCTGCCTACCTAAC | |
| Bm-Sxl E3 | Sxle3F1 | CGAGGCAGAGCGGGTTCGAAC |
| Sxle3R1 | CCTTCATCACTCGACAGCTCTC | |
| BmIMP Proximal | IMPE2F1 | ATCCTCAAAGGTACTCATCAG |
| IMPE2I2R | GCATGCATCACTCAACAATAC | |
| BmIMP Distal | IMPE7F2 | GGATCATCGGCAAAGGCGGAC |
| IMPI7R2 | AGCACTTGGATCATTCATACC | |
| EF-1 Proximal | EF-1E2F1 | TGGCGATGGAGGCGGAGAAGG |
| EF-1E2I2R | GAAAAAGAAGAAAGCATTCATGC | |
| EF-1 Distal | EF-1I11E12F | ATGATACTGTATTAACTGCATTC |
| EF-1E12R2 | TTCAGGATTTTGAGACCCTGG | |
| BmIMP E7-E8 | IMPE7F1 | ATGCGGGAAGAAGGTTTTATG |
| BmIMPR1 | TCATCCCGCCTCAGACGATTG | |
| BmIMP E1-E3 | BmIMPF1 | ATGGACGGTGACATGTCTCAAG |
| IMPE3R1 | CATCCATTCAACCCGTTTATG | |
| BmIMP E1S | BmIMPF1 | ATGGACGGTGACATGTCTCAAG |
| IMPE2R2 | GCCTGCTCTGGACTCTCGAAG | |
| BmIMP E1L | IMPE1LF1 | AATCTGCTCAAAATGGCGAAG |
| IMPE2R2 | GCCTGCTCTGGACTCTCGAAG | |
| Bm-Sxl S | BmSxlF2 | ACTCGCGTTACCTATTTAAC |
| BmSxlSR1 | GTACTGCTGTTGGATTTGGTC | |
| Bm-Sxl L | BmSxlF2 | ACTCGCGTTACCTATTTAAC |
| BmSxlLR1 | CTGCTGTTGGATTTGATTTTC |