Table 1.

The physiochemical character of the NBD protein, K71L and T204V mutants as predicted by Expasy’s Prot-Param program.

Protein	Length	M.wt (Daltons)	pI	−R	+R	Extinction coefficient (M−1·cm−1)	Instability index	Aliphatic index	GRAVY
NBD	380	41,827.4	6.69	50	49	20,525	35.09	88.32	−0.274
K71L	380	41,812.4	6.38	50	48	20,525	34.74	89.34	−0.253
T204V	380	41,825.5	6.69	50	49	20,525	34.99	89.08	−0.261