Table 3.
Validation of NBD, K71L and T204V mutants using PROCHECK and ProQ.
| Structure | Ramachandran plot statistics (%) | Goodness factor | ProQ | ||||||
|---|---|---|---|---|---|---|---|---|---|
|
|
|
|
|||||||
| Most favoured | Additionally allowed | Generously allowed | Disallowed | Dihedral angles | Covalent forces | Overall average | LG Score | Max–sub | |
| NBD | 81.7 | 15.4 | 2.1 | 0.9 | −0.61 * | −0.95 * | −0.66 * | 5.707 | 0.451 |
| K71L | 79.9 | 18.6 | 0.9 | 0.6 | −0.59 * | −1.02 ** | −0.67 * | 5.497 | 0.425 |
| T204V | 78.7 | 19.2 | 1.2 | 0.9 | −0.61 * | −0.91 * | −0.64 * | 5.862 | 0.424 |
*
Lower than −0.5-unusual;
**
Lower than −1.0-highly unusual.