Schematic diagram for the organization of Lgl gene from T. castaneum (A) and the rooted phylogenetic tree of deduced Lgl amino acid sequences from the nematode C. elegans and fourteen insect species as constructed by the neighbor-jointing method (B). All the names used in the tree consist of first letter of the genus, the first letter of the specific name followed by Lgl. Sequences used include: TcLgl (KJ434293, T. castaneum this paper); AaLgl (XP_001654112.1, A. aegypti); AfLgl (XP_003693724.1, A. florae); AgLgl (XP_313781.5, A. gambiae); AmLgl (XP_003249432.1, A. mellifera); ApLgl (XP_001948718.2, A. pisum); BmLgl (XP_004921966.1, B. mori); BtLgl (XP_003396710.1, B. terrestris); CeLgl (CCD70868.1, C. elegans); CqLgl (EDS36704.1, C. quinquefasciatus); DmLgl (AAG22255.1, D. melanogaster); HsLgl (CBH09297.1, H. melpomene); MrLgl (XP_003708503.1, M. rotundata); NvLgl (XP_001604186.2, N. vitripennis); PhLgl (EEB15731.1, P. humanus corporis).