Figure 7.

Time-dependent suppression of TcLgl transcript in early (1-day) pupae of T. castaneum injected with dsTcLgl at 100, 200, and 400 ng/pupa or dsGFP at 400 ng/pupa as determined by RT-qPCR (A); the eclosion rates in the dsTcLgl and dsGFP-treated pupae (B); and the phenotype of abnormal elcosion from the pupae injected with dsTcLgl (C). The results are presented as the mean and standard errors of three replicates; each was performed with a RNA sample prepared from four early pupae and each sample was run with two technical replicates. The percent normal eclosions were also determined based three replicates for each dsRNA dose; each replicate with at least 40 early pupae. Different letters above the standard error bars indicate significant differences based on ANOVA followed by Tukey’s HSD multiple comparison test (p < 0.05) within the same time point.