Table 1.
Primers used to amplify TcLgl cDNA sequences, synthesize dsRNA and analyze transcript levels.
| Application of Primers | Sequence (5′-3′) | Tm (°C) | Product Length (bp) | |
|---|---|---|---|---|
| PCR for cNDA sequence | TcLgl-1-F | TCGGCTGTTTTACCTGTATTTATC | 59.11 | 875 |
| TcLgl-1-R | GCCATCATTATGTGAGCTTGTG | 60.53 | ||
| TcLgl-2-F | GTGCCGTTGAGGCTATTTTT | 59.23 | 1036 | |
| TcLgl-2-R | CCGGCAACAATCAAACTTC | 59.11 | ||
| TcLgl-3-F | AGTACAGCGATGTTTTTCACTGG | 60.58 | 1030 | |
| TcLgl-3-R | CTGGAAGTGGTGCTACCCC | 60.52 | ||
| TcLgl-4-F | CTATACCTTCACGATAACGGTTCC | 60.15 | 1220 | |
| TcLgl-4-R | CTTAACACACAATTAAAAAGTTTTGGT | 58.32 | ||
|
| ||||
| dsRNA synthesis | dsGFP(T7)-F | GGATCCTAATACGACTCACTAT | 60.23 | 305 |
| AGGGTGACCACCCTGACCTAC | ||||
| dsGFP(T7)-R | GGATCCTAATACGACTCACTAT | 60.4 | ||
| AGGGTTGATGCCGTTCTTCTGC | ||||
| dsTcLgl(T7)-F | TAATACGACTCACTATAGG | 60.11 | 385 | |
| GGACGTTGCAACACGGATTC | ||||
| dsTcLgl(T7)-R | TAATACGACTCACTATAGG | 60.40 | ||
| GTGTCATCGTAAAGCTTGCCA | ||||
|
| ||||
| RT-qPCR | TcLgl(Q)-F | GACGGATGGCTTTTGCTA | 61.6 | 141 |
| TcLgl(Q)-R | CGGCATTCAACTGTCTCT | 61.2 | ||
| TcRps3-F | CCGTCGTATTCGTGAATTGACTT | 61.1 | 130 | |
| TcRps3-R | TCTAAGAGACTCTGCTTGTGCAATG | 61 | ||