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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Biochim Biophys Acta. 2014 Mar 14;1841(6):868–879. doi: 10.1016/j.bbalip.2014.03.002

Fig. 2.

Fig. 2

Expression of Cyp4f18 in bone marrow neutrophils from Cyp4f18 +/+ (WT), +/− (HZ), and −/− (KO) mice.

(A) Total RNA was extracted from neutrophils and used as a substrate for reverse transcription. The cDNA was analyzed by PCR using primer pair exon7F-exon10R.

(B) Real time PCR analysis of neutrophil cDNA using a primer-probe set that is specific for Cyp4f18 transcripts containing exons 8 and 9.

(C) Extracts of membrane proteins (20 μg) from neutrophils were analyzed by western blot. CYP4F18 protein appears as a 60 kDa band in WT extracts.