Table 1.
Summary of primers.
| PCR primers | |
|---|---|
| WTF | GTGCTGTCCATTTGTTGGTG |
| WTR | GAGCAGGAAAGGAGCTCAGA |
| KOF | GTGGTTGCCTGTGAATTCCT |
| KOR | AAGCCCATGCCTACAATGAC |
| FLF | CCACAAATGACACACCTCCA |
| FLR | TAGGCAGAATCGATGGGAAA |
| FLF2 | AGGAACTTCATCAGTCAGGTACA |
| FLR2 | CTCTCTGGGCCAAGCATATT |
| Exon7F | CTCCTTGATCAGGGTGGTGT |
| Exon10R | GGGAGCACAATGTCCTGAGT |
| Taqman primers for real time PCR (Applied Biosystems) | |
|---|---|
| Cyp4f13 | Mm00504576_m1 |
| Cyp4f14 | Mm00491623_m1 |
| Cyp4f15 | Mm00506542_m1 |
| Cyp4f16 | Mm00775893_m1 |
| Cyp4f17 | Mm01345625_m1 |
| Cyp4f18 | Mm00499348_m1 |
| Cyp4f39 | Mm00624134_m1 |
| Cyp4f40 | Mm01342246_m1 |
| Custom Taqman primers | |
|---|---|
| Cyp4f18 | (exons 8/9) |
| Forward: | AGTGGACTTTCCTGGATCCTGTAC |
| Reverse: | GGCAGCGCTCCTGGTATTC |
| Probe: | ACCTGGCAAGACACC |
| Cyp4f37 | |
| Forward: | TCCCGCCTCAGATGTTTCC |
| Reverse: | CCCAAGTGACCCAAAAACCA |
| Probe: | TCAGCCTCCTAAAAGA |
Sequences are shown in 5' to 3' direction.